Journal: Antioxidants

Article Title: Long-Term Region-Specific Mitochondrial Functionality Changes in Both Cerebral Hemispheres after fMCAo Model of Ischemic Stroke

doi: 10.3390/antiox13040416

Figure Lengend Snippet: Long-term (60, 90, 120, and 180 days after reperfusion) mitochondrial oxygen consumption changes in ( A ) cortical tissue Complex I, ( B ) hippocampal tissue Complex I, and ( C ) cortical tissue Complexes I&II; ( D ) hippocampal tissue Complexes I&II, presented as oxygen flux per volume (pmol/(s*mL)), in induced OXPHOS states in mice. Mitochondrial respiration was measured using the Oroboros O2k-FluoRespirometer, where R denotes time elapsed since reperfusion, solid lines indicate comparisons between fMCAo ipsilateral hemispheres, and dotted lines indicate comparisons between fMCAo contralateral hemispheres. Statistical comparisons were made for each test group over time and between ipsilateral and contralateral hemispheres. Significant changes over time, determined by one-way ANOVA analysis, are denoted by * p < 0.05 vs. fMCAo ipsi, R: 180 days; # p < 0.05 vs. fMCAo contra, R: 180 days; @ p < 0.05 vs. Sham ipsi, R: 180 days; and $ p < 0.05 vs. Sham contra, R: 180 days. Significant changes between hemispheres at one time-point, determined by a paired, non-parametric t -test, are denoted by f p < 0.05 vs. fMCAo contra and i p < 0.05 vs. Sham ipsi. Data are presented as mean oxygen flux per volume ± SD. Sample size for all state respirometry measures of cortical tissue: Sham 60 days: n = 10, Sham 90 days: n = 8, Sham 120 days: n = 10, Sham 180 days: n = 9; fMCAo R: 60 days: n = 12, fMCAo R: 90 days: n = 7, fMCAo R: 120 days: n = 11, fMCAo R: 180 days: n = 10. Sample of hippocampal tissue: Sham 60 days: n = 10, Sham 90 days: n = 8, Sham 120 days: n = 9, Sham 180 days: n = 10; fMCAo R: 60 days: n = 12, fMCAo R: 90 days: n = 9, fMCAo R: 120 days: n = 8, fMCAo R: 180 days: n = 9.

Article Snippet: Homogenized hemispheric cortical and hippocampal tissue samples (100 μL ± 1%) were injected into O2k chambers via Hamilton syringe (Hamilton ® Gastight ® 1700 series; Sigma-Aldrich, St. Louis, Missouri, USA).

Techniques:

Journal: Antioxidants

Article Title: Long-Term Region-Specific Mitochondrial Functionality Changes in Both Cerebral Hemispheres after fMCAo Model of Ischemic Stroke

doi: 10.3390/antiox13040416

Figure Lengend Snippet: Long-term (60, 90, 120, and 180 days after reperfusion) mitochondrial oxygen consumption changes in ( A ) cortical tissue Complex I&II, ( B ) hippocampal tissue Complex I&II, and ( C ) cortical tissue Complex II, ( D ) hippocampal tissue Complex II, presented as oxygen flux per volume (pmol/(s*mL)), during induced ET states in mice. Mitochondrial respiration was tested by using the Oroboros O2k-FluoRespirometer, where R denotes time elapsed since reperfusion, solid lines indicate comparisons between fMCAo ipsilateral hemispheres, and dotted lines indicate comparisons between fMCAo contralateral hemispheres. Statistical comparisons were made for each test group over time and between ipsilateral and contralateral hemispheres. Significant changes over time, determined by one-way ANOVA analysis, are denoted by * p < 0.05 vs. fMCAo ipsi, R: 180 days; # p < 0.05 vs. fMCAo contra, R: 180 days; @ p < 0.05 vs. Sham ipsi, R: 180 days; and $ p < 0.05 vs. Sham contra, R: 180 days. Significant changes between hemispheres at one time point, determined by a paired, non-parametric t -test, are denoted by f p < 0.05 vs. fMCAo contra; i p < 0.05 vs. Sham ipsi; and c p < 0.05 vs. Sham contra. Data are presented as mean oxygen flux per volume ± SD. Sample size for all state respirometry measures of cortical tissue are as follows: Sham 60 days: n = 10, Sham 90 days: n = 8, Sham 120 days: n = 10, Sham 180 days: n = 9; fMCAo R: 60 days: n = 12, fMCAo R: 90 days: n = 7, fMCAo R: 120 days: n = 11, fMCAo R: 180 days: n = 10. Sample of hippocampal tissue: Sham 60 days: n = 10, Sham 90 days: n = 8, Sham 120 days: n = 9, Sham 180 days: n = 10; fMCAo R: 60 days: n = 12, fMCAo R: 90 days: n = 9, fMCAo R: 120 days: n = 8, fMCAo R: 180 days: n = 9.

Article Snippet: Homogenized hemispheric cortical and hippocampal tissue samples (100 μL ± 1%) were injected into O2k chambers via Hamilton syringe (Hamilton ® Gastight ® 1700 series; Sigma-Aldrich, St. Louis, Missouri, USA).

Techniques:

Journal: Antioxidants

Article Title: Long-Term Region-Specific Mitochondrial Functionality Changes in Both Cerebral Hemispheres after fMCAo Model of Ischemic Stroke

doi: 10.3390/antiox13040416

Figure Lengend Snippet: Long-term (60, 90, 120, and 180 days after reperfusion) ( A ) cortical and ( B ) hippocampal mitochondrial ET coupling efficiencies, or uncoupler effect, with internal normalizations (expressing respiratory flux after addition of ETS uncoupler relative to respiratory flux with pyruvate, malate, ADP, glutamate, and succinate). Mitochondrial coupling efficiencies were calculated based on oxygen flux data obtained with Oroboros O2k-FluoRespirometer, where R denotes time elapsed since reperfusion, solid lines indicate comparisons between fMCAo ipsilateral hemispheres, and dotted lines indicate comparisons between fMCAo contralateral hemispheres. Statistical comparisons were made for each test group over time and between ipsilateral and contralateral hemispheres. Significant changes over time, determined by one-way ANOVA analysis, are denoted by * p < 0.05 vs. fMCAo ipsi, R: 180 days; # p < 0.05 vs. fMCAo contra, R: 180 days; and @ p < 0.05 vs. Sham ipsi, R: 180 days. Significant changes between hemispheres at one time point, determined by a paired, non-parametric t -test, are denoted by f p < 0.05 vs. fMCAo contra. Data, corrected for background noise and residual oxygen consumption, are presented as flux control ratio ± SD. Sample size: Sham 60 days: n = 10, Sham 90 days: n = 8, Sham 120 days: n = 10, Sham 180 days: n = 9; fMCAo R: 60 days: n = 12, fMCAo R: 90 days: n = 7, fMCAo R: 120 days: n = 11, fMCAo R: 180 days: n = 10. Sample of hippocampal tissue: Sham 60 days: n = 10, Sham 90 days: n = 8, Sham 120 days: n = 9, Sham 180 days: n = 10; fMCAo R: 60 days: n = 12, fMCAo R: 90 days: n = 9, fMCAo R: 120 days: n = 8, fMCAo R: 180 days: n = 9.

Article Snippet: Homogenized hemispheric cortical and hippocampal tissue samples (100 μL ± 1%) were injected into O2k chambers via Hamilton syringe (Hamilton ® Gastight ® 1700 series; Sigma-Aldrich, St. Louis, Missouri, USA).

Techniques: Expressing, Control

Journal: Antioxidants

Article Title: Long-Term Region-Specific Mitochondrial Functionality Changes in Both Cerebral Hemispheres after fMCAo Model of Ischemic Stroke

doi: 10.3390/antiox13040416

Figure Lengend Snippet: Long-term (60, 90, 120, and 180 days after reperfusion) cortical ROS emissions, presented as H 2 O 2 production normalized by tissue mass (pmol/(s*mg w.w.)), during Complex II supported states in mice ( A ) in cortical tissue LEAK state, ( B ) in cortical tissue after inhibition of Complex I with rotenone, and ( C ) in cortical tissue after inhibition of Complex III with Antimycin A. Cortical ROS emissions in mitochondria were measured using the Oroboros O2k-FluoRespirometer, where R denotes time elapsed since reperfusion, solid lines indicate comparisons between fMCAo ipsilateral hemispheres, and dotted lines indicate comparisons between fMCAo contralateral hemispheres. Statistical comparisons were made for each test group over time and between ipsilateral and contralateral hemispheres. Significant changes over time, determined by one-way ANOVA analysis, are denoted by * p < 0.05 vs. fMCAo ipsi, R: 180 days; € p < 0.05 vs. fMCAo ipsi, R: 60 days; # p < 0.05 vs. fMCAo contra, R: 180 days; + p < 0.05 vs. fMCAo contra, R: 60 days; @ p < 0.05 vs. Sham ipsi, R: 180 days; and $ p < 0.05 vs. Sham, contra R: 180 days. Significant changes between hemispheres at one time point, determined by a paired, non-parametric t -test, are denoted by f p < 0.05 vs. fMCAo contra. Data are presented as H 2 O 2 production normalized by mass (pmol/(s*mg w.w.)) ± SD. Sample size for H 2 O 2 measurements: Sham 60 days: n = 9, Sham 90 days: n = 10, Sham 120 days: n = 9, Sham 180 days: n = 10; fMCAo R: 60 days: n = 10, fMCAo R: 90 days: n = 9, fMCAo R: 120 days: n = 9, fMCAo R: 180 days: n = 12.

Article Snippet: Homogenized hemispheric cortical and hippocampal tissue samples (100 μL ± 1%) were injected into O2k chambers via Hamilton syringe (Hamilton ® Gastight ® 1700 series; Sigma-Aldrich, St. Louis, Missouri, USA).

Techniques: Inhibition

Journal: Aging (Albany NY)

Article Title: Glycocalyx-targeted therapy prevents age-related muscle loss and declines in maximal exercise capacity

doi: 10.18632/aging.206313

Figure Lengend Snippet: Mitochondrial respiration in gastrocnemius muscle of Has2 +/+ and Has2 −/− mice post-tamoxifen administration. Respiration rates were measured under various substrate conditions: State 2 (Glu + Mal), State 3 CI (ADP), State 3 CI + CII (Succ), State 3 CII (Rot), and AA + TMPD. Data are presented as mean ± SEM. * P < 0.05 vs. Has2 +/+ . N = 4–5/group.

Article Snippet: The muscle fibers were then placed in the respiration chamber (Oxygraph O2K, Oroboros Instruments, Innsbruck, Austria) with 2 ml of MiR05 solution and warmed to 37°C.

Techniques:

Journal: Archives in cancer research

Article Title: Association between Mitochondrial Bioenergetics and Radiation-Related Fatigue: A Possible Mechanism and Novel Target

doi: 10.21767/2254-6081.100014

Figure Lengend Snippet: Substrates-inhibitors tracing protocol 1 in human lymphocytes.

Article Snippet: Two milliliter of respiration buffer with intact lymphocytes will be injected to two chambers of the Oroboros-Oxygraph-2k (O2K) system.

Techniques:

Journal: Archives in cancer research

Article Title: Association between Mitochondrial Bioenergetics and Radiation-Related Fatigue: A Possible Mechanism and Novel Target

doi: 10.21767/2254-6081.100014

Figure Lengend Snippet: Substrates-inhibitors tracing protocol 2 in human lymphocytes.

Article Snippet: Two milliliter of respiration buffer with intact lymphocytes will be injected to two chambers of the Oroboros-Oxygraph-2k (O2K) system.

Techniques: